Glycoproteomics Analysis Service

Glycoproteomics explores the intricate world of protein glycosylation, assessing how sugars attach to proteins and the resultant impact on biological functions. This field is vital, particularly in biopharmaceuticals, where understanding glycosylation can significantly influence the safety and efficacy of therapies, including recombinant proteins.

 

Services at MtoZ Biolabs

At MtoZ Biolabs, we harness state-of-the-art mass spectrometry and bioinformatics to offer a robust glycoproteomics analysis service. We use both top-down and bottom-up approaches to provide a thorough characterization of glycoproteins, helping to unravel the complexities of glycosylation:

1. Top-Down: We perform direct mass spectrometric analysis on intact proteins to capture complete sequences and detailed glycan profiles.

2. Bottom-Up: This method focuses on analyzing peptides from protein digestion, allowing for precise exploration of glycopeptide structures and modifications.

 



Gabriele, C. et al. Int. J. Mol. Sci. 2021.

Figure1. Bottom-Up Based Glycoproteomics Analysis Process

 

Analysis Workflow

1. Sample Preparation: Appropriate pre-treatment based on the sample type, including protein extraction and purification.

2. Enzymatic Digestion and Glycopeptide Enrichment: Specific enzymes are used to digest proteins, followed by enrichment of glycopeptides using specialized techniques.

3. Mass Spectrometric Analysis: Detailed analysis of glycopeptides using advanced LC-ESI-MS/MS technology.

4. Data Analysis and Reporting: Professional bioinformatics processing and data interpretation provide a detailed analysis report and recommendations.

 

Service Advantages

1. Comprehensive Glycosylation Analysis

We cover all aspects of glycosylation, including both N-glycans and O-glycans, and precise glycosylation site mapping.

2. Advanced Analytical Instruments

Our facility is equipped with the latest mass spectrometry technology, ensuring depth and accuracy.

3. Professional Team

Our glycoproteomics specialists oversee each analysis step, guaranteeing data reliability and quality.

4. Rapid Response and Turnaround

We deliver swift, standard services, with options to accelerate urgent projects.

 

Sample Submission Requirements

1. Solid Samples: Such as SDS-PAGE strips, 2D gel spots.

2. Biological Fluids:  Includes animal tissues, serum, plasma, saliva.

3. Cells and Microbes: Extensive glycoprotein profiling for cellular and microbial samples.

4. Plant Materials:  Suitable for young leaves, buds, seeds, and fruits.

 

With a reputation for excellence, MtoZ Biolabs is a leading choice for glycoproteomics analysis globally. To learn more or discuss specific requirements, please reach out for personalized consultation.